Untargeted GC-TOFMS Analysis Reveals Metabolomic Changes in Salvia miltiorrhiza Bunge Leaf and Root in Response to Long-Term Drought Stress

Salvia miltiorrhiza Bunge (Danshen) is an important traditional Chinese medicine herb. This study aimed to investigate the drought-responsive metabolic profiling in S. using gas chromatography time-of-flight mass spectrometry (GC-TOFMS) analysis. Fifty day-old seedlings were treated with two (moderate drought, MD) and four weeks (high HD) of withholding water. The leaf root samples prepared for GC-TOFMS Differential metabolites substantial changes content identified multivariate univariate statistics. A total 178 157 annotated detected root, respectively. Multivariate analysis showed that significantly discriminant by drought associated “galactose metabolism” “citrate cycle”. In addition, “starch sucrose metabolism”. Univariate statistics succinic acid, d-glucose, oxoglutaric acid was increased (fold change, FC > 1.5). Allose, d-xylose, melibiose, mannose, sorbitol, quinic sinigrin, taurine decreased (FC < 0.67). There different profiles between root. However, influence stress on pharmacological value accumulation bioactive constituents should be further investigated.